Review



patr s1989  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc patr s1989
    Patr S1989, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/Phospho-ATR+(Thr1989)+Rabbit+mAb/pmc12682131-4-0-5
    Average 95 stars, based on 86 article reviews
    patr s1989 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: DDB2 expression lights the way for precision radiotherapy response in PDAC cells, with or without olaparib
    Article Snippet: .. The primary antibodies mouse anti-α-Tubulin (cat# sc-23948, Santa Cruz Biotechnology, 1:1000), mouse anti-Chk1 (cat# 2360S, Cell Signaling, Danvers, MA, USA, 1:1000), rabbit anti-pChk1 (cat# 2344S, Cell Signaling, 1:500), rabbit anti-Chk2 (cat# 6334S, Cell Signaling, 1:1000), rabbit anti-pChk2 (cat# 2197S, Cell Signaling, 1:1000), rabbit anti-ATM (cat# 2873S, Cell Signaling, 1:1000), rabbit anti-pATM (cat# 5883S, Cell Signaling, 1:500), rabbit anti-ATR (cat# 13934S, Cell Signaling, 1:1000), rabbit anti-pATR (cat# 2853S, Cell Signaling, 1:1000), mouse anti-PARP (#cat 556362, BD Biosciences, 1:1000), rabbit anti-cleaved PARP (#cat 5625S, Cell Signaling, 1:1000), and rabbit anti-Poly/mono-ADP Ribose (cat#83732S, Cell Signaling, 1:1000) were incubated in blocking solution overnight at 4 °C. .. Secondary goat anti-rabbit HRP-linked (cat# 7074S, Cell Signaling, 1:2000) and horse anti-mouse HRP-linked (cat# 7076S, Cell Signaling, 1:2000) antibodies were applied for one hour at room temperature.

    Article Title: DDB2 expression lights the way for precision radiotherapy response in PDAC cells, with or without olaparib.
    Article Snippet: .. The primary antibodies mouse anti-α-Tubulin (cat# sc-23948, Santa Cruz Biotechnology, 1:1000), mouse anti-Chk1 (cat# 2360S, Cell Signaling, Danvers, MA, USA, 1:1000), rabbit anti-pChk1 (cat# 2344S, Cell Signaling, 1:500), rabbit anti-Chk2 (cat# 6334S, Cell Signaling, 1:1000), rabbit anti-pChk2 (cat# 2197S, Cell Signaling, 1:1000), rabbit anti-ATM (cat# 2873S, Cell Signaling, 1:1000), rabbit anti-pATM (cat# 5883S, Cell Signaling, 1:500), rabbit anti-ATR (cat# 13934S, Cell Signaling, 1:1000), rabbit anti-pATR (cat# 2853S, Cell Signaling, 1:1000), mouse anti-PARP (#cat 556362, BD Biosciences, 1:1000), rabbit anti-cleaved PARP (#cat 5625S, Cell Signaling, 1:1000), and rabbit antiPoly/mono-ADP Ribose (cat#83732S, Cell Signaling, 1:1000) were incubated in blocking solution overnight at 4 °C. .. Secondary goat anti-rabbit HRP-linked (cat# 7074S, Cell Signaling, 1:2000) and horse anti-mouse HRP-linked (cat# 7076S, Cell Signaling, 1:2000) antibodies were applied for one hour at room temperature.

    Blocking Assay:

    Article Title: DDB2 expression lights the way for precision radiotherapy response in PDAC cells, with or without olaparib
    Article Snippet: .. The primary antibodies mouse anti-α-Tubulin (cat# sc-23948, Santa Cruz Biotechnology, 1:1000), mouse anti-Chk1 (cat# 2360S, Cell Signaling, Danvers, MA, USA, 1:1000), rabbit anti-pChk1 (cat# 2344S, Cell Signaling, 1:500), rabbit anti-Chk2 (cat# 6334S, Cell Signaling, 1:1000), rabbit anti-pChk2 (cat# 2197S, Cell Signaling, 1:1000), rabbit anti-ATM (cat# 2873S, Cell Signaling, 1:1000), rabbit anti-pATM (cat# 5883S, Cell Signaling, 1:500), rabbit anti-ATR (cat# 13934S, Cell Signaling, 1:1000), rabbit anti-pATR (cat# 2853S, Cell Signaling, 1:1000), mouse anti-PARP (#cat 556362, BD Biosciences, 1:1000), rabbit anti-cleaved PARP (#cat 5625S, Cell Signaling, 1:1000), and rabbit anti-Poly/mono-ADP Ribose (cat#83732S, Cell Signaling, 1:1000) were incubated in blocking solution overnight at 4 °C. .. Secondary goat anti-rabbit HRP-linked (cat# 7074S, Cell Signaling, 1:2000) and horse anti-mouse HRP-linked (cat# 7076S, Cell Signaling, 1:2000) antibodies were applied for one hour at room temperature.

    Article Title: DDB2 expression lights the way for precision radiotherapy response in PDAC cells, with or without olaparib.
    Article Snippet: .. The primary antibodies mouse anti-α-Tubulin (cat# sc-23948, Santa Cruz Biotechnology, 1:1000), mouse anti-Chk1 (cat# 2360S, Cell Signaling, Danvers, MA, USA, 1:1000), rabbit anti-pChk1 (cat# 2344S, Cell Signaling, 1:500), rabbit anti-Chk2 (cat# 6334S, Cell Signaling, 1:1000), rabbit anti-pChk2 (cat# 2197S, Cell Signaling, 1:1000), rabbit anti-ATM (cat# 2873S, Cell Signaling, 1:1000), rabbit anti-pATM (cat# 5883S, Cell Signaling, 1:500), rabbit anti-ATR (cat# 13934S, Cell Signaling, 1:1000), rabbit anti-pATR (cat# 2853S, Cell Signaling, 1:1000), mouse anti-PARP (#cat 556362, BD Biosciences, 1:1000), rabbit anti-cleaved PARP (#cat 5625S, Cell Signaling, 1:1000), and rabbit antiPoly/mono-ADP Ribose (cat#83732S, Cell Signaling, 1:1000) were incubated in blocking solution overnight at 4 °C. .. Secondary goat anti-rabbit HRP-linked (cat# 7074S, Cell Signaling, 1:2000) and horse anti-mouse HRP-linked (cat# 7076S, Cell Signaling, 1:2000) antibodies were applied for one hour at room temperature.

    other:

    Article Title: Initiation of Genome Instability and Preneoplastic Processes through Loss of Fhit Expression
    Article Snippet: Images were acquired with a Zeiss Axioskop 40 fluorescent microscope mounted with an AxioCam HRc camera, and using an A-Plan 10×/0.25 objective lens.



    Similar Products

    patr s1989  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc patr s1989
    Patr S1989, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/Phospho-ATR+(Thr1989)+Rabbit+mAb/pmc12682131-4-0-5
    Average 95 stars, based on 1 article reviews
    patr s1989 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    rabbit anti patr thr1989  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc rabbit anti patr thr1989
    A , B Impact of COL1 binding and niraparib at 1 µM on activation of ATR and ATM in W1 and W1CR cells after 24 h, analyzed by Western Blotting. A and B display the relative expressions of p-ATR <t>(Thr1989)</t> and p-ATM (Ser1981) normalized on untreated W1 cells. Protein bands of a representative Western Blot and the respective total protein bands of the stainfree visualization or the housekeeping protein band of β -actin are presented below the graphs. Data represent means ± SD ( n = 3). To test for significance paired t -tests were applied for cultivation and unpaired t -tests for cell line comparison. * P < 0.05; ** P < 0.01. C LC 50 values of niraparib cytotoxicity in W1 and W1CR cells, treated with ATR or ATM inhibitors at the indicated concentrations. All cells were cultivated on COL1. Data represent means ± SD ( n = 7). Statistical analysis was performed by paired t -tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Violin plot of log 2 fold changes between cytotoxicity of solo (niraparib) and combined (niraparib + ATRi/ATMi) treated W1 and W1CR cells cultivated on COL1. Lines in violins represent median and quartiles ( n = 7). Asterisks display the significance levels calculated in C and in Supplement Fig. . E Quantification of the protein amount of phosphorylated H2AX (Ser139) as a marker for damaged DNA under the indicated treatments in W1 and W1CR cells either cultivated on COL1 or not. Data represent means ± SD ( n = 3). Statistical analysis was performed by One-Way ANOVA following Dunnett’s test. * P < 0.05.
    Rabbit Anti Patr Thr1989, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/Phospho-ATR+(Thr1989)+Rabbit+mAb/pmc12501025-208-3-14
    Average 95 stars, based on 1 article reviews
    rabbit anti patr thr1989 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    patr t1989  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc patr t1989
    A , B Impact of COL1 binding and niraparib at 1 µM on activation of ATR and ATM in W1 and W1CR cells after 24 h, analyzed by Western Blotting. A and B display the relative expressions of p-ATR <t>(Thr1989)</t> and p-ATM (Ser1981) normalized on untreated W1 cells. Protein bands of a representative Western Blot and the respective total protein bands of the stainfree visualization or the housekeeping protein band of β -actin are presented below the graphs. Data represent means ± SD ( n = 3). To test for significance paired t -tests were applied for cultivation and unpaired t -tests for cell line comparison. * P < 0.05; ** P < 0.01. C LC 50 values of niraparib cytotoxicity in W1 and W1CR cells, treated with ATR or ATM inhibitors at the indicated concentrations. All cells were cultivated on COL1. Data represent means ± SD ( n = 7). Statistical analysis was performed by paired t -tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Violin plot of log 2 fold changes between cytotoxicity of solo (niraparib) and combined (niraparib + ATRi/ATMi) treated W1 and W1CR cells cultivated on COL1. Lines in violins represent median and quartiles ( n = 7). Asterisks display the significance levels calculated in C and in Supplement Fig. . E Quantification of the protein amount of phosphorylated H2AX (Ser139) as a marker for damaged DNA under the indicated treatments in W1 and W1CR cells either cultivated on COL1 or not. Data represent means ± SD ( n = 3). Statistical analysis was performed by One-Way ANOVA following Dunnett’s test. * P < 0.05.
    Patr T1989, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/Phospho-ATR+(Thr1989)+Rabbit+mAb/bio_rxiv__2025__07__17__665300-119-3-25
    Average 95 stars, based on 1 article reviews
    patr t1989 - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    rabbit monoclonal anti patr  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc rabbit monoclonal anti patr
    A , B Impact of COL1 binding and niraparib at 1 µM on activation of ATR and ATM in W1 and W1CR cells after 24 h, analyzed by Western Blotting. A and B display the relative expressions of p-ATR <t>(Thr1989)</t> and p-ATM (Ser1981) normalized on untreated W1 cells. Protein bands of a representative Western Blot and the respective total protein bands of the stainfree visualization or the housekeeping protein band of β -actin are presented below the graphs. Data represent means ± SD ( n = 3). To test for significance paired t -tests were applied for cultivation and unpaired t -tests for cell line comparison. * P < 0.05; ** P < 0.01. C LC 50 values of niraparib cytotoxicity in W1 and W1CR cells, treated with ATR or ATM inhibitors at the indicated concentrations. All cells were cultivated on COL1. Data represent means ± SD ( n = 7). Statistical analysis was performed by paired t -tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Violin plot of log 2 fold changes between cytotoxicity of solo (niraparib) and combined (niraparib + ATRi/ATMi) treated W1 and W1CR cells cultivated on COL1. Lines in violins represent median and quartiles ( n = 7). Asterisks display the significance levels calculated in C and in Supplement Fig. . E Quantification of the protein amount of phosphorylated H2AX (Ser139) as a marker for damaged DNA under the indicated treatments in W1 and W1CR cells either cultivated on COL1 or not. Data represent means ± SD ( n = 3). Statistical analysis was performed by One-Way ANOVA following Dunnett’s test. * P < 0.05.
    Rabbit Monoclonal Anti Patr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/Phospho-ATR+(Thr1989)+Rabbit+mAb/pm40240600-1003-75-80
    Average 95 stars, based on 1 article reviews
    rabbit monoclonal anti patr - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    rabbit monoclonal anti patr 75 cell signaling technology  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc rabbit monoclonal anti patr 75 cell signaling technology
    A , B Impact of COL1 binding and niraparib at 1 µM on activation of ATR and ATM in W1 and W1CR cells after 24 h, analyzed by Western Blotting. A and B display the relative expressions of p-ATR <t>(Thr1989)</t> and p-ATM (Ser1981) normalized on untreated W1 cells. Protein bands of a representative Western Blot and the respective total protein bands of the stainfree visualization or the housekeeping protein band of β -actin are presented below the graphs. Data represent means ± SD ( n = 3). To test for significance paired t -tests were applied for cultivation and unpaired t -tests for cell line comparison. * P < 0.05; ** P < 0.01. C LC 50 values of niraparib cytotoxicity in W1 and W1CR cells, treated with ATR or ATM inhibitors at the indicated concentrations. All cells were cultivated on COL1. Data represent means ± SD ( n = 7). Statistical analysis was performed by paired t -tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Violin plot of log 2 fold changes between cytotoxicity of solo (niraparib) and combined (niraparib + ATRi/ATMi) treated W1 and W1CR cells cultivated on COL1. Lines in violins represent median and quartiles ( n = 7). Asterisks display the significance levels calculated in C and in Supplement Fig. . E Quantification of the protein amount of phosphorylated H2AX (Ser139) as a marker for damaged DNA under the indicated treatments in W1 and W1CR cells either cultivated on COL1 or not. Data represent means ± SD ( n = 3). Statistical analysis was performed by One-Way ANOVA following Dunnett’s test. * P < 0.05.
    Rabbit Monoclonal Anti Patr 75 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/Phospho-ATR+(Thr1989)+Rabbit+mAb/pmc12074999__41586_2025_8835_MOESM1_ESM-131-84-88
    Average 95 stars, based on 1 article reviews
    rabbit monoclonal anti patr 75 cell signaling technology - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    antibody anti-patr (thr1989) (rabbit polyclonal)  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc antibody anti-patr (thr1989) (rabbit polyclonal)

    Antibody Anti Patr (Thr1989) (Rabbit Polyclonal), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+patr/anti+phospho+atr++thr1989+/pmc11996177-21-2-7
    Average 90 stars, based on 1 article reviews
    antibody anti-patr (thr1989) (rabbit polyclonal) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A , B Impact of COL1 binding and niraparib at 1 µM on activation of ATR and ATM in W1 and W1CR cells after 24 h, analyzed by Western Blotting. A and B display the relative expressions of p-ATR (Thr1989) and p-ATM (Ser1981) normalized on untreated W1 cells. Protein bands of a representative Western Blot and the respective total protein bands of the stainfree visualization or the housekeeping protein band of β -actin are presented below the graphs. Data represent means ± SD ( n = 3). To test for significance paired t -tests were applied for cultivation and unpaired t -tests for cell line comparison. * P < 0.05; ** P < 0.01. C LC 50 values of niraparib cytotoxicity in W1 and W1CR cells, treated with ATR or ATM inhibitors at the indicated concentrations. All cells were cultivated on COL1. Data represent means ± SD ( n = 7). Statistical analysis was performed by paired t -tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Violin plot of log 2 fold changes between cytotoxicity of solo (niraparib) and combined (niraparib + ATRi/ATMi) treated W1 and W1CR cells cultivated on COL1. Lines in violins represent median and quartiles ( n = 7). Asterisks display the significance levels calculated in C and in Supplement Fig. . E Quantification of the protein amount of phosphorylated H2AX (Ser139) as a marker for damaged DNA under the indicated treatments in W1 and W1CR cells either cultivated on COL1 or not. Data represent means ± SD ( n = 3). Statistical analysis was performed by One-Way ANOVA following Dunnett’s test. * P < 0.05.

    Journal: Cell Death Discovery

    Article Title: Increased efficacy of PARP inhibitors against cisplatin-sensitive and -resistant ovarian cancer cells mediated via ATR and ATM inhibition

    doi: 10.1038/s41420-025-02740-1

    Figure Lengend Snippet: A , B Impact of COL1 binding and niraparib at 1 µM on activation of ATR and ATM in W1 and W1CR cells after 24 h, analyzed by Western Blotting. A and B display the relative expressions of p-ATR (Thr1989) and p-ATM (Ser1981) normalized on untreated W1 cells. Protein bands of a representative Western Blot and the respective total protein bands of the stainfree visualization or the housekeeping protein band of β -actin are presented below the graphs. Data represent means ± SD ( n = 3). To test for significance paired t -tests were applied for cultivation and unpaired t -tests for cell line comparison. * P < 0.05; ** P < 0.01. C LC 50 values of niraparib cytotoxicity in W1 and W1CR cells, treated with ATR or ATM inhibitors at the indicated concentrations. All cells were cultivated on COL1. Data represent means ± SD ( n = 7). Statistical analysis was performed by paired t -tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. D Violin plot of log 2 fold changes between cytotoxicity of solo (niraparib) and combined (niraparib + ATRi/ATMi) treated W1 and W1CR cells cultivated on COL1. Lines in violins represent median and quartiles ( n = 7). Asterisks display the significance levels calculated in C and in Supplement Fig. . E Quantification of the protein amount of phosphorylated H2AX (Ser139) as a marker for damaged DNA under the indicated treatments in W1 and W1CR cells either cultivated on COL1 or not. Data represent means ± SD ( n = 3). Statistical analysis was performed by One-Way ANOVA following Dunnett’s test. * P < 0.05.

    Article Snippet: Therefore, we used rabbit anti-pATR (Thr1989) (#30632S), rabbit anti-pATM (Ser1981) (#13050 T) (purchased from Cell Signaling Technology, Frankfurt am Main, Germany), mouse anti-PARP1 (#sc-56197), mouse anti-α-tubulin (#sc-8035), (purchased from Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-GAPDH (#T0004; GeneTex, Irvine, CA, USA) diluted in TBS-T containing 1% BSA and 0.05% sodium azide.

    Techniques: Binding Assay, Activation Assay, Western Blot, Comparison, Marker

    Journal: eLife

    Article Title: Treacle’s ability to form liquid-like phase condensates is essential for nucleolar fibrillar center assembly, efficient rRNA transcription and processing, and rRNA gene repair

    doi: 10.7554/eLife.96722

    Figure Lengend Snippet:

    Article Snippet: Antibody , Anti-pATR (Thr1989) (Rabbit polyclonal) , Cell Signaling , 58014, RRID: AB_2722679 , ChIP-qPCR.

    Techniques: In Situ, Imaging, Software